4p5h

X-ray diffraction
3.38Å resolution

Structure of Clostridium perfringens Enterotoxin with a peptide derived from a modified version of ECL-2 of Claudin 2

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Claudin-2 Chains: 1, 2, 3, 4, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, 3, 4, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 20 amino acids
Theoretical weight: 2.38 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: O88552 (Residues: 141-160; Coverage: 9%)
Gene name: Cldn2
Heat-labile enterotoxin B chain Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 286 amino acids
Theoretical weight: 31.52 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P01558 (Residues: 38-319; Coverage: 88%)
Gene name: cpe
Sequence domains: Clostridium enterotoxin
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 369.6Å b: 100.26Å c: 265.36Å
α: 90° β: 119.74° γ: 90°
R-values:
R R work R free
0.206 0.204 0.24
Expression systems:
  • Not provided
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'