› 4p4n

X-ray diffraction
1.95Å resolution

Crystal Structure of Mycobacterium tuberculosis Shikimate Dehydrogenase

Released:
DOI: 10.2210/pdb4p4n/pdb
PDB 4p4n coloured by chain and viewed from the front.
PDB 4p4n coloured by chain and viewed from the side.
PDB 4p4n coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis
Entry authors: Lalgondar M, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 277 amino acids
Theoretical weight: 28.29 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6Y120 (Residues: 1-269; Coverage: 100%)
Gene names: Rv2552c, aroE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 128.568Å b: 132.696Å c: 38.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.229
Expression system: Escherichia coli BL21(DE3)

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