4p4g

X-ray diffraction
1.7Å resolution

Crystal Structure of Mycobacterium tuberculosis Shikimate Dehydrogenase

Released:
Source organism: Mycobacterium tuberculosis
Entry authors: Lalgondar M, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed:
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 28.29 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6Y120 (Residues: 1-269; Coverage: 100%)
Gene names: Rv2552c, aroE
Sequence domains: Shikimate dehydrogenase substrate binding domain
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 43.467Å b: 75.553Å c: 129.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.228
Expression system: Escherichia coli BL21(DE3)