4p3q

X-ray diffraction
1.35Å resolution

Room-temperature WT DHFR, time-averaged ensemble

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli str. K-12 substr. DH10B
Expression system: Escherichia coli
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 34.32Å b: 45.51Å c: 98.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.153
Expression system: Escherichia coli