› 4p3j

X-ray diffraction
3.5Å resolution

Apo inward-facing state of the glutamate transporter homologue GltPh in alkali-free conditions

Released:
DOI: 10.2210/pdb4p3j/pdb
PDB entry 4p3j coloured by chain, front view.
PDB entry 4p3j coloured by chain, side view.
PDB entry 4p3j coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Coupled ion binding and structural transitions along the transport cycle of glutamate transporters.
Verdon G, Oh S, Serio RN, Boudker O
Elife 3 e02283 (2014)
PMID: 24842876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.62 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

1 bound ligand:
Ligand HG 3 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 106.948Å b: 196.843Å c: 207.484Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.263 0.278
Expression system: Escherichia coli K-12

Quick links

Citations

11 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
10 more

5 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
4 more