› 4p33

X-ray diffraction
1.65Å resolution

Crystal structure of E. coli LptB-E163Q in complex with ATP-sodium

Released:
DOI: 10.2210/pdb4p33/pdb
PDB entry 4p33 coloured by chain, front view.
PDB entry 4p33 coloured by chain, side view.
PDB entry 4p33 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport.
Sherman DJ, Lazarus MB, Murphy L, Liu C, Walker S, Ruiz N, Kahne D
Proc Natl Acad Sci U S A 111 4982-7 (2014)
PMID: 24639492

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-141930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipopolysaccharide export system ATP-binding protein LptB Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli KRX
UniProt:
  • Canonical: P0A9V1 (Residues: 2-241; Coverage: 100%)
Gene names: JW3168, b3201, lptB, yhbG
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand ATP 2 x ATP
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 66.62Å b: 138.36Å c: 101.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.21
Expression system: Escherichia coli KRX

Quick links

Citations

14 review citations

Function and Biogenesis of Lipopolysaccharides.
Bertani et al. (2018)
13 more

6 mentions without citation

Lipopolysaccharide transport to the cell surface: biosynthesis and extraction from the inner membrane.
Simpson et al. (2015)
5 more