4p0a

X-ray diffraction
2.3Å resolution

Crystal structure of HOIP PUB domain in complex with p97 PIM

Released:

Function and Biology Details

Reactions catalysed:
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
ATP + H(2)O = ADP + phosphate
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase RNF31 Chains: A, C
Molecule details ›
Chains: A, C
Length: 184 amino acids
Theoretical weight: 20.77 KDa
Source organism: Homo sapiens
Expression system: Bacteria
UniProt:
  • Canonical: Q96EP0 (Residues: 1-179; Coverage: 17%)
Gene names: RNF31, ZIBRA
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Transitional endoplasmic reticulum ATPase Chains: B, D
Molecule details ›
Chains: B, D
Length: 10 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P55072 (Residues: 797-806; Coverage: 1%)
Gene name: VCP

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P61
Unit cell:
a: 48.04Å b: 48.04Å c: 266.622Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.249
Expression systems:
  • Bacteria
  • Not provided