› 4ozo

X-ray diffraction
2.6Å resolution

Crystal structure of an a-L-fucosidase GH29 from Bacteroides thetaiotaomicron (BT2192) in complex with oNPTG

Released:
DOI: 10.2210/pdb4ozo/pdb
PDB entry 4ozo coloured by chain, front view.
PDB entry 4ozo coloured by chain, side view.
PDB entry 4ozo coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Unraveling the substrate recognition mechanism and specificity of the unusual glycosyl hydrolase family 29 BT2192 from Bacteroides thetaiotaomicron.
Guillotin L, Lafite P, Daniellou R
Biochemistry 53 1447-55 (2014)
PMID: 24527659

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-linkages between alpha-L-fucose and N-acetylglucosamine residues in glycoproteins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F5/8 type C domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 469 amino acids
Theoretical weight: 53.42 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A5P6 (Residues: 26-483; Coverage: 99%)
Gene name: BT_2192
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand OTN 2 x OTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I212121
Unit cell:
a: 125.3Å b: 122.17Å c: 159.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
1 more

3 mentions without citation

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis.
Fang et al. (2021)
2 more