X-ray diffraction
1.95Å resolution

Crystal structure of porcine aminopeptidase N complexed with CNGRCG tumor-homing peptide

Source organism: Sus scrofa
Entry authors: Liu C, Yang Y, Chen L, Lin Y-L, Li F

Function and Biology Details

Reaction catalysed:
Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Aminopeptidase N Chain: A
Molecule details ›
Chain: A
Length: 908 amino acids
Theoretical weight: 103.52 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
  • Canonical: P15145 (Residues: 63-963; Coverage: 94%)
Gene name: ANPEP
Sequence domains:
Structure domains:
tumor-homing peptide Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 610 Da
Source organism: Sus scrofa
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 260.323Å b: 62.879Å c: 82.023Å
α: 90° β: 100.59° γ: 90°
R R work R free
0.143 0.14 0.19
Expression systems:
  • Spodoptera frugiperda
  • Not provided