4otz

X-ray diffraction
1.36Å resolution

The crystal structure of a solute-binding protein (N280D mutant) from Anabaena variabilis ATCC 29413 in complex with cystein

Released:
Entry authors: Tan K, Li H, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peripla_BP_6 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 42.36 KDa
Source organism: Trichormus variabilis ATCC 29413
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3MFZ5 (Residues: 25-416; Coverage: 99%)
Gene name: Ava_0465
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 98.738Å b: 100.952Å c: 150.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.139 0.167
Expression system: Escherichia coli