4ot1

X-ray diffraction
2.11Å resolution

Structural Basis for the Recognition of Human Cytomegalovirus Glycoprotein B by the Neutralizing Human Antibody SM5-1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein B Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.43 KDa
Source organism: Human herpesvirus 5 strain Towne
Expression system: Escherichia coli
UniProt:
  • Best match: P13201 (Residues: 114-133, 344-438)
Gene names: UL55, gB
SM5-1 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
SM5-1 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 43.071Å b: 69.411Å c: 100.991Å
α: 90° β: 99.68° γ: 90°
R-values:
R R work R free
0.174 0.171 0.231
Expression systems:
  • Escherichia coli
  • Not provided