› 4opl

X-ray diffraction
2.51Å resolution

Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.

Released:
DOI: 10.2210/pdb4opl/pdb
PDB 4opl coloured by chain and viewed from the front.
PDB 4opl coloured by chain and viewed from the side.
PDB 4opl coloured by chain and viewed from the top.
Source organism: Sulfolobus acidocaldarius DSM 639
Primary publication:
Constructing Tailored Isoprenoid Products by Structure-Guided Modification of Geranylgeranyl Reductase.
Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD
Structure (2014)
PMID: 24954619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Digeranylgeranylglycerophospholipid reductase Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 50.86 KDa
Source organism: Sulfolobus acidocaldarius DSM 639
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4JA33 (Residues: 1-452; Coverage: 100%)
Gene name: Saci_0986
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

1 bound ligand:
Ligand FDA 1 x FDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 65.331Å b: 78.84Å c: 105.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.259 0.292
Expression system: Escherichia coli

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Citations

6 review citations

Leveraging microbial biosynthetic pathways for the generation of 'drop-in' biofuels.
Zargar et al. (2017)
5 more