4oor

X-ray diffraction
2.7Å resolution

Ancestral Steroid Receptor 2 DNA binding domain in complex with a steroid response element

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Ancestral Steroid Receptor 2 DNA binding domain Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.04 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3' Chains: I, K
Molecule details ›
Chains: I, K
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3' Chains: J, L
Molecule details ›
Chains: J, L
Length: 18 nucleotides
Theoretical weight: 5.47 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 47.472Å b: 80.374Å c: 115.645Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.193 0.19 0.233
Expression systems:
  • Escherichia coli
  • Not provided