X-ray diffraction
2.3Å resolution

Clp1 bound to ssRNA dinucleotide GC, ADP, AlF4-, and Mg2+(transition state, data set II)


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein clpf-1 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.94 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P52874 (Residues: 1-428; Coverage: 100%)
Gene names: F59A2.4, clpf-1
Sequence domains: mRNA cleavage and polyadenylation factor CLP1 P-loop
Structure domains:
RNA (5'-R(*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 2 nucleotides
Theoretical weight: 605 Da
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P31
Unit cell:
a: 100.74Å b: 100.74Å c: 40.41Å
α: 90° β: 90° γ: 120°
R R work R free
0.192 0.19 0.242
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided