› 4ohn

X-ray diffraction
1.37Å resolution

Crystal structure of an ABC uptake transporter substrate binding protein from Streptococcus pneumoniae with Bound Histidine

Released:
DOI: 10.2210/pdb4ohn/pdb
PDB 4ohn coloured by chain and viewed from the front.
PDB 4ohn coloured by chain and viewed from the side.
PDB 4ohn coloured by chain and viewed from the top.
Source organism: Streptococcus pneumoniae str. Canada MDR_19A
Entry authors: Brunzelle JS, Wawrzak W, Yim Y, Kudritska M, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PBPb domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.66 KDa
Source organism: Streptococcus pneumoniae str. Canada MDR_19A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UKY8 (Residues: 1-257; Coverage: 100%)
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand HIS 1 x HIS
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 87.125Å b: 87.125Å c: 70.949Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.137 0.153
Expression system: Escherichia coli BL21(DE3)

Quick links