4ohn

X-ray diffraction
1.37Å resolution

Crystal structure of an ABC uptake transporter substrate binding protein from Streptococcus pneumoniae with Bound Histidine

Released:
Entry authors: Brunzelle JS, Wawrzak W, Yim Y, Kudritska M, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PBPb domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.66 KDa
Source organism: Streptococcus pneumoniae str. Canada MDR_19A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UKY8 (Residues: 1-257; Coverage: 100%)
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 87.125Å b: 87.125Å c: 70.949Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.137 0.153
Expression system: Escherichia coli BL21(DE3)