PDBe 4oh8

X-ray diffraction
2.28Å resolution

Crystal Structure of the human MST1-RASSF5 SARAH heterodimer

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase 4 18kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 6.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13043 (Residues: 432-480; Coverage: 10%)
Gene names: KRS2, MST1, STK4
Sequence domains: C terminal SARAH domain of Mst1
Ras association domain-containing protein 5 Chain: B
Molecule details ›
Chain: B
Length: 55 amino acids
Theoretical weight: 6.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WWW0 (Residues: 366-418; Coverage: 13%)
Gene names: NORE1, RAPL, RASSF5
Sequence domains: Novel Ras effector 1 C-terminal SARAH (Sav/Rassf/Hpo) domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: C2221
Unit cell:
a: 27.94Å b: 85.84Å c: 92.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.278
Expression system: Escherichia coli BL21