PDBe 4ogy

X-ray diffraction
2.1Å resolution

Crystal structure of Fab DX-2930 in complex with human plasma kallikrein at 2.1 Angstrom resolution

Released:

Function and Biology Details

Reaction catalysed:
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Plasma kallikrein light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03952 (Residues: 391-631; Coverage: 39%)
Gene names: KLK3, KLKB1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
IgG H chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 225 amino acids
Theoretical weight: 24.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: S6C4R2 (Residues: 20-244; Coverage: 88%)
Sequence domains:
Structure domains: Immunoglobulins
DX-2930 LIGHT CHAIN Chains: L, N
Molecule details ›
Chains: L, N
Length: 213 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 82.12Å b: 113.76Å c: 89.05Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.185 0.183 0.221
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli