4ocq

X-ray diffraction
1.88Å resolution

N-acetylhexosamine 1-phosphate kinase in complex with GalNAc

Released:
Source organism: Bifidobacterium longum
Entry authors: Li TL, Wang KC, Lyu SY, Liu YC, Chang CY, Wu CJ

Function and Biology Details

Reaction catalysed:
ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylhexosamine 1-kinase Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 42.1 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli
UniProt:
  • Canonical: E8MF12 (Residues: 1-359; Coverage: 100%)
Gene names: BLLJ_1622, lnpB, nahK
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 55.212Å b: 79.623Å c: 98.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.218
Expression system: Escherichia coli