› 4oan

X-ray diffraction
1.35Å resolution

Crystal structure of a TRAP periplasmic solute binding protein from rhodopseudomonas palustris HaA2 (RPB_2686), TARGET EFI-510221, with density modeled as (S)-2-hydroxy-2-methyl-3-oxobutanoate ((S)-2-Acetolactate)

Released:
DOI: 10.2210/pdb4oan/pdb
PDB entry 4oan coloured by chain, front view.
PDB entry 4oan coloured by chain, side view.
PDB entry 4oan coloured by chain, top view.
Source organism: Rhodopseudomonas palustris HaA2
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP dicarboxylate transporter DctP subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 37.36 KDa
Source organism: Rhodopseudomonas palustris HaA2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2IWM2 (Residues: 30-339; Coverage: 100%)
Gene name: RPB_2686
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand X2X 2 x X2X
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 55.064Å b: 59.609Å c: 61.005Å
α: 62.62° β: 71.24° γ: 67.18°
R-values:
R R work R free
0.144 0.143 0.162
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
12 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)