4o9u

X-ray diffraction
6.93Å resolution

Mechanism of transhydrogenase coupling proton translocation and hydride transfer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proton-translocating NAD(P)(+) transhydrogenase Chains: A, C
Molecule details ›
Chains: A, C
Length: 100 amino acids
Theoretical weight: 10.65 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GR9 (Residues: 1-100; Coverage: 100%)
Gene name: TT_C1779
Sequence domains: 4TM region of pyridine nucleotide transhydrogenase, mitoch
NAD(P) transhydrogenase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 450 amino acids
Theoretical weight: 47.37 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GS0 (Residues: 1-450; Coverage: 100%)
Gene name: TT_C1778
Sequence domains: NAD(P) transhydrogenase beta subunit
NAD/NADP transhydrogenase alpha subunit 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 384 amino acids
Theoretical weight: 41.26 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GR8 (Residues: 1-375; Coverage: 100%)
Gene name: TT_C1780
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 175.331Å b: 160.59Å c: 139.624Å
α: 90° β: 128.36° γ: 90°
R-values:
R R work R free
0.216 0.213 0.284
Expression system: Escherichia coli