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› 4o9c

X-ray diffraction
2Å resolution

Crystal structure of Beta-ketothiolase (PhaA) from Ralstonia eutropha H16

Released:
DOI: 10.2210/pdb4o9c/pdb
PDB entry 4o9c coloured by chain, front view.
PDB entry 4o9c coloured by chain, side view.
PDB entry 4o9c coloured by chain, top view.
Source organism: Cupriavidus necator H16
Primary publication:
Crystal structure and biochemical characterization of PhaA from Ralstonia eutropha, a polyhydroxyalkanoate-producing bacterium.
Kim EJ, Kim KJ
Biochem Biophys Res Commun 452 124-9 (2014)
PMID: 25152395

Function and Biology Details

Reaction catalysed: 
[2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA., 2 acetyl-CoA = acetoacetyl-CoA + CoA.]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 393 amino acids
Theoretical weight: 40.58 KDa
Source organism: Cupriavidus necator H16
Expression system: Escherichia coli
UniProt:
  • Canonical: P14611 (Residues: 1-393; Coverage: 100%)
Gene names: H16_A1438, phaA, phbA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 1 x COA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 102.092Å b: 157.438Å c: 114.867Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.213
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system.
Wu et al. (2019)
2 more

1 mention without citation

Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase from Ralstonia eutropha.
Fage et al. (2015)