4o7f

X-ray diffraction
1.8Å resolution

Crystal structure of the first bromodomain of human BRD4 in complex with SB-251527

Released:
Source organism: Homo sapiens
Entry authors: Ember SW, Zhu J-Y, Watts C, Schonbrunn E

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 41.869Å b: 59.464Å c: 109.606Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.14 0.194
Expression system: Escherichia coli BL21(DE3)