› 4o6n

X-ray diffraction
2.1Å resolution

Structure of AF2299, a CDP-alcohol phosphotransferase (CDP-bound)

Released:
DOI: 10.2210/pdb4o6n/pdb
PDB 4o6n coloured by chain and viewed from the front.
PDB 4o6n coloured by chain and viewed from the side.
PDB 4o6n coloured by chain and viewed from the top.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structural basis for catalysis in a CDP-alcohol phosphotransferase.
Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F
OpenAccess logo Nat Commun 5 4068 (2014)
PMID: 24923293

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AF2299, a CDP-alcohol phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 42.26 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O27985 (Residues: 1-344; Coverage: 100%)
Gene name: AF_2299
Sequence domains: CDP-alcohol phosphatidyltransferase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 4 x CA
Ligand CDP 2 x CDP
Ligand MPG 9 x MPG
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 46.329Å b: 90.67Å c: 106.444Å
α: 90° β: 92.07° γ: 90°
R-values:
R R work R free
0.228 0.226 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

From yeast to humans - roles of the Kennedy pathway for phosphatidylcholine synthesis.
McMaster CR. (2018)
2 more

3 mentions without citation

Lipid topogenesis--35years on.
Chauhan et al. (2016)
2 more