PDBe 4o6f

X-ray diffraction
2.82Å resolution

Structural Basis of Estrogen Receptor Alpha Methylation Mediated by Histone Methyltransferase SmyD2

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-lysine methyltransferase SMYD2 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 49.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NRG4 (Residues: 1-433; Coverage: 100%)
Gene names: KMT3C, SMYD2
Sequence domains:
Structure domains:
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03372 (Residues: 261-271; Coverage: 2%)
Gene names: ESR, ESR1, NR3A1

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I4
Unit cell:
a: 151.81Å b: 151.81Å c: 52.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.236
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided