4o6a

X-ray diffraction
1.86Å resolution

Mouse cyclic GMP-AMP synthase (cGAS) in complex with DNA

Released:

Function and Biology Details

Reaction catalysed:
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 42.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene names: Cgas, Mb21d1
Sequence domains: Mab-21 protein
Structure domains:
DNA1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 17 nucleotides
Theoretical weight: 5.16 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 183.711Å b: 96.33Å c: 75.989Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.187 0.186 0.218
Expression systems:
  • Escherichia coli
  • Not provided