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Crystal Structure of SMARCAL1 HARP substrate recognition domain

X-ray diffraction
1.9Å resolution
PDB entry 4o66 coloured by chain, front view.
PDB entry 4o66 coloured by chain, side view.
PDB entry 4o66 coloured by chain, top view.
Source organism: Mus musculus
Assemblies composition:
monomeric
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: monomeric
Accessible surface area: 5394.62 Å2
Buried surface area: 151.64 Å2
Dissociation area: 75.82 Å2
Dissociation energy (ΔGdiss): 11.51 kcal/mol
Dissociation entropy (TΔSdiss): 0.58 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183879
Assembly 2
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: monomeric
Accessible surface area: 5385.41 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183879
Assembly 3
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: monomeric
Accessible surface area: 5911.42 Å2
Buried surface area: 460.27 Å2
Dissociation area: 55.38 Å2
Dissociation energy (ΔGdiss): 7 kcal/mol
Dissociation entropy (TΔSdiss): 0.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183879
Assembly 4
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: monomeric
Accessible surface area: 5593.49 Å2
Buried surface area: 128.41 Å2
Dissociation area: 64.2 Å2
Dissociation energy (ΔGdiss): 11.05 kcal/mol
Dissociation entropy (TΔSdiss): -1.11 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183879
Assembly 5
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: homo dimer
Accessible surface area: 7210.08 Å2
Buried surface area: 3721.59 Å2
Dissociation area: 1,788.55 Å2
Dissociation energy (ΔGdiss): 38.29 kcal/mol
Dissociation entropy (TΔSdiss): 10.93 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-183880
Assembly 6 (preferred)
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Assembly Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
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Multimeric state: homo dimer
Accessible surface area: 7214.02 Å2
Buried surface area: 4879.57 Å2
Dissociation area: 2,138.3 Å2
Dissociation energy (ΔGdiss): 53.27 kcal/mol
Dissociation entropy (TΔSdiss): 11.15 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-183880

Macromolecules

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Chains: A, B, C, D
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8BJL0 (Residues: 197-268; Coverage: 8%)
Gene names: Harp, Smarcal1
Pfam: HepA-related protein (HARP)
InterPro: HARP domain
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 in PDB entry 4o66, assembly 6, front view.
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 in PDB entry 4o66, assembly 6, side view.
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 in PDB entry 4o66, assembly 6, top view.
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