4o22

X-ray diffraction
1.7Å resolution

Binary complex of metal-free PKAc with SP20.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 40.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 16-351; Coverage: 96%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: S
Molecule details ›
Chain: S
Length: 20 amino acids
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61925 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 56.63Å b: 97.86Å c: 78.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.219
Expression system: Escherichia coli