› 4o1s

X-ray diffraction
2.7Å resolution

Crystal structure of TvoVMA intein

Released:
DOI: 10.2210/pdb4o1s/pdb
PDB entry 4o1s coloured by chain, front view.
PDB entry 4o1s coloured by chain, side view.
PDB entry 4o1s coloured by chain, top view.
Source organism: Thermoplasma volcanium GSS1
Primary publication:
Structure-based engineering and comparison of novel split inteins for protein ligation.
Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwaï H
Mol Biosyst 10 1023-34 (2014)
PMID: 24574026

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tvo AtpA intein Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 18.56 KDa
Source organism: Thermoplasma volcanium GSS1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97CQ0 (Residues: 234-421; Coverage: 22%)
Gene names: TV0051, TVG0054274, atpA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

3 bound ligands:
Ligand EPE 2 x EPE
Ligand MRD 1 x MRD
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P63
Unit cell:
a: 154.442Å b: 154.442Å c: 48.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.23
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cyclic Peptides: Promising Scaffolds for Biopharmaceuticals.
Gang et al. (2018)
2 more

5 mentions without citation

Nature's recipe for splitting inteins.
Aranko et al. (2014)
4 more