PDBe 4nyj

X-ray diffraction
2.85Å resolution

Approach for Targeting Ras with Small Molecules that Activate SOS-Mediated Nucleotide Exchange

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GTPase HRas, N-terminally processed Chain: Q
Molecule details ›
Chain: Q
Length: 166 amino acids
Theoretical weight: 18.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Son of sevenless homolog 1 Chain: S
Molecule details ›
Chain: S
Length: 481 amino acids
Theoretical weight: 56.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07889 (Residues: 566-1046; Coverage: 36%)
Gene name: SOS1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I422
Unit cell:
a: 185.338Å b: 185.338Å c: 179.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.187
Expression system: Escherichia coli