› 4nx2

X-ray diffraction
2Å resolution

Crystal structure of DCYRS complexed with DCY

Released:
DOI: 10.2210/pdb4nx2/pdb
PDB entry 4nx2 coloured by chain, front view.
PDB entry 4nx2 coloured by chain, side view.
PDB entry 4nx2 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Significant expansion of fluorescent protein sensing ability through the genetic incorporation of superior photo-induced electron-transfer quenchers.
Liu X, Jiang L, Li J, Wang L, Yu Y, Zhou Q, Lv X, Gong W, Lu Y, Wang J
J Am Chem Soc 136 13094-7 (2014)
PMID: 25197956

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.71 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 2LT 1 x 2LT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 53.099Å b: 160.955Å c: 39.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.22
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.
Jin et al. (2019)
5 more

1 mention without citation

Halogenation of tyrosine perturbs large-scale protein self-organization.
Sun et al. (2022)