4nv7

X-ray diffraction
2.02Å resolution

Crystal Structure of Mesorhizobium Loti Arylamine N-acetyltransferase 1 In Complex With CoA

Released:
Entry authors: Xu XM, Haouz A, Weber P, Li de la sierra-gallay I, Kubiak X, Dupret J-M, Rodrigues-lima F

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arylamine N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 34.36 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q98D42 (Residues: 1-278; Coverage: 100%)
Gene name: mlr4870
Sequence domains: N-acetyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 53.5Å b: 114.86Å c: 115.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.221
Expression system: Escherichia coli BL21