X-ray diffraction
2.5Å resolution

Crystal structure of a transition state mimic of the GSK-3/Axin complex bound to phosphorylated N-terminal auto-inhibitory pS9 peptide


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycogen synthase kinase-3 beta Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 44.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: Q9WV60 (Residues: 1-383; Coverage: 91%)
Gene name: Gsk3b
Sequence domains: Protein kinase domain
Structure domains:
Axin-1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O15169 (Residues: 383-402; Coverage: 2%)
Gene names: AXIN, AXIN1

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6122
Unit cell:
a: 80.997Å b: 80.997Å c: 280.53Å
α: 90° β: 90° γ: 120°
R R work R free
0.194 0.191 0.245
Expression system: Escherichia coli