› 4nty

X-ray diffraction
2.65Å resolution

Cesium sites in the crystal structure of acid-sensing ion channel in complex with snake toxin

Released:
DOI: 10.2210/pdb4nty/pdb
PDB entry 4nty coloured by chain, front view.
PDB entry 4nty coloured by chain, side view.
PDB entry 4nty coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structure of acid-sensing ion channel 1-snake toxin complex reveals open state of a Na(+)-selective channel.
Baconguis I, Bohlen CJ, Goehring A, Julius D, Gouaux E
Cell 156 717-29 (2014)
PMID: 24507937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-124673 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Acid-sensing ion channel 1 Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51.33 KDa
Source organism: Gallus gallus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q1XA76 (Residues: 14-463; Coverage: 85%)
Gene names: ACCN2, ASIC1
Sequence domains: Amiloride-sensitive sodium channel
Structure domains:
Kunitz-type neurotoxin MitTx-alpha Chain: B
Molecule details ›
Chain: B
Length: 60 amino acids
Theoretical weight: 7.12 KDa
Source organism: Micrurus tener tener
Expression system: Escherichia coli
UniProt:
  • Canonical: G9I929 (Residues: 25-84; Coverage: 100%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Basic phospholipase A2 homolog MitTx-beta Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.77 KDa
Source organism: Micrurus tener tener
Expression system: Escherichia coli
UniProt:
  • Canonical: G9I930 (Residues: 31-149; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CS 15 x CS
Ligand CL 1 x CL
Ligand PE4 1 x PE4
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R3
Unit cell:
a: 151.53Å b: 151.53Å c: 123.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.255
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

37 review citations

International Union of Basic and Clinical Pharmacology. XCI. structure, function, and pharmacology of acid-sensing ion channels and the epithelial Na+ channel.
Kellenberger et al. (2015)
36 more

6 mentions without citation

Mechanisms of Action of the Peptide Toxins Targeting Human and Rodent Acid-Sensing Ion Channels and Relevance to Their In Vivo Analgesic Effects.
Verkest et al. (2022)
5 more