› 4nsd

X-ray diffraction
2.7Å resolution

Crystal Structure of CBARA1 in the Ca2+ Binding Form

Released:
DOI: 10.2210/pdb4nsd/pdb
PDB entry 4nsd coloured by chain, front view.
PDB entry 4nsd coloured by chain, side view.
PDB entry 4nsd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake.
Wang L, Yang X, Li S, Wang Z, Liu Y, Feng J, Zhu Y, Shen Y
EMBO J 33 594-604 (2014)
PMID: 24514027

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uptake protein 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 40.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BPX6 (Residues: 97-444; Coverage: 73%)
Gene names: CALC, CBARA1, MICU1
Sequence domains: EF hand

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand CL 2 x CL
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I212121
Unit cell:
a: 99.283Å b: 105.804Å c: 156.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.282
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Calcium at the Center of Cell Signaling: Interplay between Endoplasmic Reticulum, Mitochondria, and Lysosomes.
Raffaello et al. (2016)
27 more

8 mentions without citation

Mitochondrial Ca2+ transport in the endothelium: regulation by ions, redox signalling and mechanical forces.
Alevriadou et al. (2017)
7 more