4nro

X-ray diffraction
2.3Å resolution

Crystal structure of human ALKBH5 in complex with alpha-ketoglutarate

Released:

Function and Biology Details

Reaction catalysed:
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA demethylase ALKBH5 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P6C2 (Residues: 66-292; Coverage: 58%)
Gene names: ABH5, ALKBH5, OFOXD1
Structure domains: Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 56.177Å b: 56.177Å c: 144.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.237 0.256
Expression system: Escherichia coli