› 4nog

X-ray diffraction
1.2Å resolution

Crystal structure of a putative ornithine aminotransferase from Toxoplasma gondii ME49 in complex with pyrodoxal-5'-phosphate

Released:
DOI: 10.2210/pdb4nog/pdb
PDB entry 4nog coloured by chain, front view.
PDB entry 4nog coloured by chain, side view.
PDB entry 4nog coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii.
Lykins JD, Filippova EV, Halavaty AS, Minasov G, Zhou Y, Dubrovska I, Flores KJ, Shuvalova LA, Ruan J, El Bissati K, Dovgin S, Roberts CW, Woods S, Moulton JD, Moulton H, McPhillie MJ, Muench SP, Fishwick CWG, Sabini E, Shanmugam D, Roos DS, McLeod R, Anderson WF, Ngô HM
Front Cell Infect Microbiol 8 352 (2018)
PMID: 30345257

Function and Biology Details

Reaction catalysed: 
L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 49.03 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S8EY38 (Residues: 17-441; Coverage: 96%)
Gene name: TGME49_269110
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
4 bound ligands:
Ligand BME 2 x BME
Ligand ACT 1 x ACT
Ligand BTB 1 x BTB
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 56.19Å b: 61.328Å c: 63.678Å
α: 100.58° β: 93.23° γ: 107.74°
R-values:
R R work R free
0.135 0.133 0.165
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Treatment of toxoplasmosis: Current options and future perspectives.
Konstantinovic et al. (2019)
3 more

2 mentions without citation

CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii.
Lykins et al. (2018)
1 more