4nmy

X-ray diffraction
1.9Å resolution

Crystal Structure of the Thiamin-bound form of Substrate-binding Protein of ABC Transporter from Clostridium difficile

Released:
Source organism: Clostridioides difficile 630
Entry authors: Kim Y, Zhou M, Grimshaw S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NMT1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 34.36 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q187U0 (Residues: 38-337; Coverage: 97%)
Gene name: CD630_19790
Sequence domains: NMT1/THI5 like
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 49.529Å b: 76.867Å c: 154.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.203
Expression system: Escherichia coli BL21(DE3)