4nmf

X-ray diffraction
1.95Å resolution

Crystal structure of proline utilization A (PutA) from Geobacter sulfurreducens PCA inactivated by N-propargylglycine and complexed with menadione bisulfite

Released:
Source organism: Geobacter sulfurreducens PCA
Entry authors: Singh H, Tanner JJ

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PutA Chains: A, B
Molecule details ›
Chains: A, B
Length: 1005 amino acids
Theoretical weight: 112.32 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q746X3 (Residues: 1-1004; Coverage: 100%)
Gene names: GSU3395, putA
Sequence domains:

Ligands and Environments


Cofactor: Ligand P5F 2 x P5F
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 95.594Å b: 151.741Å c: 176.606Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.203
Expression system: Escherichia coli BL21