› 4nkt

X-ray diffraction
1.9Å resolution

Structure of Cid1 in complex with the UTP analog UMPNPP

Released:
DOI: 10.2210/pdb4nkt/pdb
PDB entry 4nkt coloured by chain, front view.
PDB entry 4nkt coloured by chain, side view.
PDB entry 4nkt coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
A critical switch in the enzymatic properties of the Cid1 protein deciphered from its product-bound crystal structure.
Munoz-Tello P, Gabus C, Thore S
Nucleic Acids Res 42 3372-80 (2014)
PMID: 24322298

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 38.98 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O13833 (Residues: 40-377; Coverage: 84%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 2KH 2 x 2KH
Ligand BR 3 x BR
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 53.77Å b: 77.32Å c: 82.11Å
α: 90° β: 90.86° γ: 90°
R-values:
R R work R free
0.18 0.179 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Functions and mechanisms of RNA tailing by metazoan terminal nucleotidyltransferases.
Liudkovska et al. (2021)
1 more

3 mentions without citation

A critical switch in the enzymatic properties of the Cid1 protein deciphered from its product-bound crystal structure.
Munoz-Tello et al. (2014)
2 more