X-ray diffraction
2.29Å resolution

Crystal Structure of the periplasmic alginate epimerase AlgG D317A mutant


Function and Biology Details

Reaction catalysed:
(Mannuronan)-beta-D-mannuronate = (alginate)-alpha-L-guluronate
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Mannuronan C5-epimerase Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 55.09 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
  • Canonical: Q887Q3 (Residues: 46-536; Coverage: 98%)
Gene names: PSPTO_1238, algG
Sequence domains: Right handed beta helix region
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 125.599Å b: 125.599Å c: 98.487Å
α: 90° β: 90° γ: 120°
R R work R free
0.21 0.208 0.236
Expression system: Escherichia coli