› 4njr

X-ray diffraction
2.3Å resolution

Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4njr/pdb
PDB 4njr coloured by chain and viewed from the front.
PDB 4njr coloured by chain and viewed from the side.
PDB 4njr coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa.
Nguyen DD, Pandian R, Kim D, Ha SC, Yoon HJ, Kim KS, Yun KH, Kim JH, Kim KK
Biochem. Biophys. Res. Commun. (2014)
PMID: 24704201

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable M18 family aminopeptidase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 429 amino acids
Theoretical weight: 46.71 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYZ3 (Residues: 1-429; Coverage: 100%)
Gene names: PA3247, apeB
Sequence domains: Aminopeptidase I zinc metalloprotease (M18)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand CO3 4 x CO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: R3
Unit cell:
a: 134.187Å b: 134.187Å c: 328.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.146 0.196
Expression system: Escherichia coli

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Citations

1 review citation

The extracellular vesicle generation paradox: a bacterial point of view.
McMillan et al. (2021)
7 other articles