X-ray diffraction
2.22Å resolution

crystal structure of cofactor(NAD+) bound 3-phosphoglycerate dehydrogenase in Entamoeba histolytica


Function and Biology Details

Reaction catalysed:
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
D-phosphoglycerate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.75 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
  • Canonical: Q76KF5 (Residues: 1-299; Coverage: 100%)
Gene names: EHI_060860, EhPGDH
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

Cofactor: Ligand NAD 1 x NAD
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 49.378Å b: 56.857Å c: 57.259Å
α: 66.71° β: 66.22° γ: 77.36°
R R work R free
0.203 0.201 0.246
Expression system: Escherichia coli