4nia

X-ray diffraction
1.82Å resolution

Satellite Tobacco Mosaic Virus Refined at room temperature to 1.8 A Resolution using NCS Restraints

Released:
Primary publication:
Satellite tobacco mosaic virus refined to 1.4 Å resolution.
Acta Crystallogr. D Biol. Crystallogr. 70 2316-30 (2014)
PMID: 25195746

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
Coat protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 159 amino acids
Theoretical weight: 17.53 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
Structure domains: Satellite virus coat domain
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d
Molecule details ›
Chains: P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: e, f, g, h, i, j, k, l, m, n, o, p, q, r, s
Molecule details ›
Chains: e, f, g, h, i, j, k, l, m, n, o, p, q, r, s
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*U)-3') Chains: 1, 2, 3, 4, 5, 6, 7, 8, t, u, v, w, x, y, z
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, t, u, v, w, x, y, z
Length: 2 nucleotides
Theoretical weight: 567 Da
Source organism: Satellite tobacco mosaic virus

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 174.268Å b: 191.774Å c: 202.503Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.156