X-ray diffraction
2Å resolution

Structure of the spliceosomal DEAD-box protein Prp28

Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28.
Acta Crystallogr. D Biol. Crystallogr. 70 1622-30 (2014)
PMID: 24914973

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Probable ATP-dependent RNA helicase DDX23 Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 55.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9BUQ8 (Residues: 338-820; Coverage: 59%)
Gene name: DDX23
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 125.43Å b: 136.77Å c: 73.22Å
α: 90° β: 90° γ: 90°
R R work R free
0.194 0.193 0.218
Expression system: Escherichia coli BL21(DE3)