4ng2

X-ray diffraction
2.41Å resolution

Crystal structure of LasR LBD-QslA complex from Pseudomonas aeruginosa

Released:
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Fan H, Wu DH, Song H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional activator protein LasR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 184 amino acids
Theoretical weight: 20.63 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P25084 (Residues: 1-170; Coverage: 71%)
Gene names: PA1430, lasR
Sequence domains: Autoinducer binding domain
Structure domains: Transcription factor LuxR-like, autoinducer-binding domain
QslA_E domain-containing protein Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 113 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I494 (Residues: 1-113; Coverage: 100%)
Gene name: PA1244
Sequence domains: LasR-specific antiactivator QslA chain E

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 163.507Å b: 185.887Å c: 56.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.277
Expression system: Escherichia coli