4nfy

X-ray diffraction
2.45Å resolution

Crystal Structure of 3-phosphoglycerate Dehydrogenase from Entamoeba histolytica

Released:

Function and Biology Details

Reaction catalysed:
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-3-phosphoglycerate dehydrogenase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.59 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76KF5 (Residues: 1-299; Coverage: 100%)
Gene names: EHI_060860, EhPGDH
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41
Unit cell:
a: 85.906Å b: 85.906Å c: 84.492Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.259
Expression system: Escherichia coli