X-ray diffraction
1.85Å resolution

Human Aprataxin (Aptx) bound to DNA, AMP, and Zn - product complex


Function and Biology Details

Reactions catalysed:
(DNA)-3'-diphospho-5'-guanosine + H(2)O = (DNA)-3'-phosphate + GMP
Adenosine-5'-diphospho-5'-(DNA) + H(2)O = AMP + phospho-5'-(DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Aprataxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q7Z2E3 (Residues: 179-356; Coverage: 50%)
  • Best match: Q7Z2E3-2 (Residues: 1-168)
Gene names: APTX, AXA1
Sequence domains:
Structure domains: HIT-like
5'-D(P*GP*TP*TP*CP*TP*AP*GP*AP*AP*C)-3' Chains: D, E, G, H
Molecule details ›
Chains: D, E, G, H
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 40.397Å b: 117.082Å c: 118.202Å
α: 90° β: 90° γ: 90°
R R work R free
0.164 0.162 0.196
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided