4nco

X-ray diffraction
4.7Å resolution

Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Complex with the Broadly Neutralizing Fab PGT122

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein gp160 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 475 amino acids
Theoretical weight: 53.12 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-504; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
BG505 SOSIP gp41 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 78 amino acids
Theoretical weight: 6.66 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
PGT122 light chain Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 211 amino acids
Theoretical weight: 22.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT122 heavy chain Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 235 amino acids
Theoretical weight: 25.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 152.2Å b: 260.72Å c: 283.18Å
α: 90° β: 99.56° γ: 90°
R-values:
R R work R free
0.376 0.375 0.389
Expression system: Homo sapiens