› 4nbt

X-ray diffraction
1.48Å resolution

Crystal structure of FabG from Acholeplasma laidlawii

Released:
DOI: 10.2210/pdb4nbt/pdb
PDB entry 4nbt coloured by chain, front view.
PDB entry 4nbt coloured by chain, side view.
PDB entry 4nbt coloured by chain, top view.
Source organism: Acholeplasma laidlawii PG-8A
Primary publication:
Biochemical and structural studies of NADH-dependent FabG used to increase the bacterial production of fatty acids under anaerobic conditions.
Javidpour P, Pereira JH, Goh EB, McAndrew RP, Ma SM, Friedland GD, Keasling JD, Chhabra SR, Adams PD, Beller HR
Appl Environ Microbiol 80 497-505 (2014)
PMID: 24212572

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 240 amino acids
Theoretical weight: 25.53 KDa
Source organism: Acholeplasma laidlawii PG-8A
Expression system: Escherichia coli
UniProt:
  • Canonical: A9NFJ2 (Residues: 1-240; Coverage: 100%)
Gene names: ACL_0504, fabG2
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 61.968Å b: 62.364Å c: 66.765Å
α: 66.03° β: 76.63° γ: 79.93°
R-values:
R R work R free
0.128 0.128 0.154
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Metabolic engineering strategies for microbial synthesis of oleochemicals.
Pfleger et al. (2015)
6 more

7 mentions without citation

Ultrafast Dynamics of Plasmon-Exciton Interaction of Ag Nanowire- Graphene Hybrids for Surface Catalytic Reactions.
Ding et al. (2016)
6 more