› 4na2

X-ray diffraction
2.3Å resolution

Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase bound to its natural intermediate

Released:
DOI: 10.2210/pdb4na2/pdb
PDB entry 4na2 coloured by chain, front view.
PDB entry 4na2 coloured by chain, side view.
PDB entry 4na2 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
A close look at a ketosynthase from a trans-acyltransferase modular polyketide synthase.
Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT
Structure 22 444-51 (2014)
PMID: 24508341

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase PksJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 637 amino acids
Theoretical weight: 70.6 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40806 (Residues: 3336-3953; Coverage: 12%)
Gene names: BSU17180, pksJ, pksK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 165.12Å b: 71.34Å c: 116.47Å
α: 90° β: 95.1° γ: 90°
R-values:
R R work R free
0.213 0.21 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Biosynthesis of polyketides by trans-AT polyketide synthases.
Helfrich et al. (2016)
12 more

6 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
5 more